BiQ Analyzer - a software tool for DNA methylation analysis
BiQ Analyzer is a software tool for easy visualization and quality control of DNA methylation data from bisulfite sequencing.
Highlights:
- End-to-end support of the analysis
process: from raw sequence files to a comprehensive documentation and visualization
- Automatically generate publication-quality
lollipop diagrams (show example)
- Integrated 1-click multiple sequence
alignment
- Automated CpG highlighting- never spend your time highlighting CpGs by hand anymore
- Open electropherogram files to check for sequencing problems (requires an electropherogram viewer such as Chromas LITE)
- Generate MethDB-compatible DNA methylation files for database submission
- Factor 5 speedup of sequence
analysis while at the same time achieving better data quality
Intended users:
- Anyone who works with DNA methylation
data from bisulfite sequencing
- Occasional users as well as experts
(the former will benefit from the help that the program gives in order to
achieve a good quality management whereas the latter will save hours and days
of tedious work)
Details on methodology
See our Applications Note that appeared in Bioinformatics (Oxford University Press).
A few more reasons to use BiQ Analyzer:
How to proceed from here:
If you consider using BiQ Analyzer,
we strongly suggest that you proceed as follows (this should not take more than
30 minutes and it might save you a lot of time later on):
- Step through the Guided
Tour / Exemplary Analysis, where we show commented sceenshots of an examplary
analysis.
- Download
and install the program as described in the
Installation section
- Reproduce the analysis from the
Guided Tour yourself, using the demonstration data that we provide. This is
the fastest way to understand how the program works and how it can be used
to speed up data analysis.
- Apply it to your own dataset!
- Look into the FAQ
in case there is any unexpected behavior of the program.
- If you have any problems or suggestions,
please write an e-mail to biq-analyzer@bioproject.de.
Typical screenshot of BiQ Analyzer in action:
Please see
References
on how to cite BiQ Analyzer
